Therapeutic Asset Ratings

Autologous Cd19-Cd20-Nkg2D-Nsbicephali Car-T Cell

Autologous Cd30.Car-T

Autologous CD34+ cells isolated from mobilised peripheral blood by positive selection, modified by CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) mediated gene editing consisting of a guide RNA (gRNA) introduced transiently as ribonucleoprotein (RNP) complex, targeting the erythroid lineage-specific enhancer region of BCL11A (B-cell lymphoma/leukemia 11A). The site-specific cleavage by Cas9 forms a double strand break (DSB), which is subsequently repaired by nonhomologous end-joining (NHEJ), leading to the transcriptional repression of BCL11A, a repressor of ?-globin gene transcription

autologous CD3+ enriched T lymphocytes obtained from peripheral blood mononuclear cells (PBMCs) by apheresis. The cells are transduced with a self-inactivating, non-replicating lentiviral vector encoding an enhanced-affinity T cell receptor (TCR) targeting the HLA-A*02:01-restricted MAGE-A4 peptide (GVYDGREHTV) alongside the CD8 alpha (CD8?) co-receptor. The expressed transgene comprises the coding sequence for the CD8? co-receptor, separated by a foot-and-mouth disease virus 2A self-cleaving peptide sequence from the coding sequence of the MAGE-A4 T-cell receptor alpha and beta chains that are separated from each other by a porcine teschovirus-1 2A self-cleaving peptide sequence, under control of the human elongation factor 1 alpha (EF-1?) promoter. The construct is flanked by 5' and 3' long terminal repeats (LTRs) and also contains a tRNA primer binding site, a ? packaging signal, a truncated gag, a Rev response element (RRE) and a central polypurine tract (cPPT) sequence 5' to the transgene, and a polypurine tract (PPT) 3' to the transgene. The vector is pseudotyped with the vesicular stomatitis virus (VSV) G envelope glycoprotein. The CD3+ T lymphocytes are purified and activated using anti-CD3/anti-CD28 monoclonal antibody coated magnetic beads. The cells are then cultured in media containing interleukin 2 (IL-2), human AB serum and a protein kinase B (AKT inhibitor). The cells are CD3+ (> 80%), express the high affinity MAGE-A4 specific TCR and CD8? co-receptor (CD8? +TCR+; >16%) and are cytotoxic to MAGE-A4 expressing tumor cells

autologous CD3+ enriched T lymphocytes obtained from peripheral blood mononuclear cells (PBMCs) by apheresis. The cells are transduced with a self-inactivating, non-replicating lentiviral vector encoding an enhanced-affinity T cell receptor (TCR) targeting the HLA-A*02:01-restricted MAGE-A4 peptide (GVYDGREHTV) alongside the CD8 alpha (CD8?) co-receptor. The expressed transgene comprises the coding sequence for the CD8? co-receptor, separated by a foot-and-mouth disease virus 2A self-cleaving peptide sequence from the coding sequence of the MAGE-A4 T-cell receptor alpha and beta chains that are separated from each other by a porcine teschovirus-1 2A self-cleaving peptide sequence, under control of the human elongation factor 1 alpha (EF-1?) promoter. The construct is flanked by 5' and 3' long terminal repeats (LTRs) and also contains a tRNA primer binding site, a ? packaging signal, a truncated gag, a Rev response element (RRE) and a central polypurine tract (cPPT) sequence 5' to the transgene, and a polypurine tract (PPT) 3' to the transgene. The vector is pseudotyped with the vesicular stomatitis virus (VSV) G envelope glycoprotein. The CD3+ T lymphocytes are purified and activated using anti-CD3/anti-CD28 monoclonal antibody coated magnetic beads. The cells are then cultured in media containing interleukin 2 (IL-2), human AB serum and a protein kinase B (AKT inhibitor). The cells are CD3+ (> 80%), express the high affinity MAGE-A4 specific TCR and CD8? co-receptor (CD8? +TCR+; >16%) and are cytotoxic to MAGE-A4 expressing tumor cells

autologous CD3+ enriched T lymphocytes obtained from peripheral blood mononuclear cells (PBMCs) by apheresis. The cells are transduced with a self-inactivating, non-replicating lentiviral vector encoding an enhanced-affinity T cell receptor (TCR) targeting the HLA-A*02:01-restricted MAGE-A4 peptide (GVYDGREHTV) alongside the CD8 alpha (CD8?) co-receptor. The expressed transgene comprises the coding sequence for the CD8? co-receptor, separated by a foot-and-mouth disease virus 2A self-cleaving peptide sequence from the coding sequence of the MAGE-A4 T-cell receptor alpha and beta chains that are separated from each other by a porcine teschovirus-1 2A self-cleaving peptide sequence, under control of the human elongation factor 1 alpha (EF-1?) promoter. The construct is flanked by 5' and 3' long terminal repeats (LTRs) and also contains a tRNA primer binding site, a ? packaging signal, a truncated gag, a Rev response element (RRE) and a central polypurine tract (cPPT) sequence 5' to the transgene, and a polypurine tract (PPT) 3' to the transgene. The vector is pseudotyped with the vesicular stomatitis virus (VSV) G envelope glycoprotein. The CD3+ T lymphocytes are purified and activated using anti-CD3/anti-CD28 monoclonal antibody coated magnetic beads. The cells are then cultured in media containing interleukin 2 (IL-2), human AB serum and a protein kinase B (AKT inhibitor). The cells are CD3+ (> 80%), express the high affinity MAGE-A4 specific TCR and CD8? co-receptor (CD8? +TCR+; >16%) and are cytotoxic to MAGE-A4 expressing tumor cells

AUTOLOGOUS CD4+ AND CD8+ T CELLS, ENRICHED FROM PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMCS) OBTAINED BY APHERESIS TRANSDUCED EX VIVO WITH A NON-REPLICATING, SELF-INACTIVATING (SIN) LENTIVIRAL VECTOR, ENCODING A CHIMERIC ANTIGEN RECEPTOR (CAR) CONSISTING OF AN ANTI-CD19 SPECIFIC SCFV, A CD8.ALPHA. STALK, TRANSMEMBRANE DOMAIN AND ANCHOR, A 41BB ENDODOMAIN AND A CD3 ZETA ENDODOMAIN, UNDER THE CONTROL OF A HUMAN PGK1 PROMOTER AND A MODIFIED WOODCHUCK POST TRANSCRIPTIONAL RESPONSE ELEMENT. THE VECTOR GENOME ALSO CONTAINS A 5' TERMINAL CMV ENHANCER/PROMOTER, A .PSI. PACKAGING SIGNAL, A REV RESPONSE ELEMENT (RRE) AND A CENTRAL POLYPURINE TRACT (CPPT)

Autologous Dcs Derived From Pbmc Loaded With Rna Encoding The Human Cmv Matrix Protein Pp65-Fllamp Plus Gm-Csf

Autologous Dcs Derived From Pbmc Loaded With Rna Encoding The Human Cmv Matrix Protein Pp65-Fllamp Plus Gm-Csf

Autologous Fl-33 Car T Therapy

Autologous Genetically Modified Mage-A4ᶜ¹º³²T Cells

Autologous Genetically Modified Mage-A4ᶜ¹º³²T Cells

Autologous Genetically Modified Mage-A4ᶜ¹º³²T Cells

Autologous Genetically Modified Mage-A4ᶜ¹º³²T Cells

AUTOLOGOUS LEUKOCYTES, 89ZR-OXINE LABELED

AUTOLOGOUS LEUKOCYTES, 89ZR-OXINE LABELED

Autologous Mesothelin-Specific Tcr-T Cells

Autologous Muc1-Activated T-Cells

Autologous Muc1-Activated T-Cells

Autologous Muc1-Activated T-Cells

Autologous Muc1-Activated T-Cells

Autologous Muc1-Activated T-Cells

Autologous Muc1-Activated T-Cells

Autologous Muc1-Activated T-Cells

Autologous Natural Killer / Natural Killer T Cell Immunotherapy